All-trans-retinoic acid: measurement
نویسندگان
چکیده
A quantitative determination of physiological levels of all-trans-retinoic acid (vitamin A acid) in human serum has been developed. A double-phase extraction of 3.5 ml of serum followed by a specific and sensitive high performance liquid chromatography procedure allowed measurement of levels down to 1 ng/ml. Serum concentrations in 37 fasting volunteers ranged from 2.7 to 4.2 ng/ml and fitted a normalGaussian distributional shape with a mean value of 3.5 ng/ml and SD of 0.4 ng/ml, as demonstrated by the KolmogorovSmirnov test.-De Leenheer, A. P., W. E. Lambert, and I. Claeys. All-trans-retinoic acid: measurement of reference values in human serum by high performance liquid chromatography. J. Lipid Res. 1982. 23: 1362-1367 Supplementary key words vitamin A acid biological fluids All-trans-retinoic acid (RA, vitamin A acid), a physiological metabolite of retinol ( l ) , retains the growthpromoting and epithelial-differentiating activity of retinol (2, 3); however it cannot maintain reproduction (4) or vision (5). A highly specific retinoic acid binding protein (CRABP) isolated from rat tissues (6, 7) and chicken embryo skin (8, 9) was demonstrated to be elevated in human lung and breast tumors (1 0-1 2). This suggests a biochemical function for RA in the cell proliferating process (1 3). Furthermore, retinoic acid and its aromatic analogs inhibit both the incidence and the severity of chemically induced tumors (14-16). Up to now, a thorough study of the biochemical role of retinoic acid suffered from the lack of suitable analytical techniques (1 7). Consequently, retinoic acid was measured in biological materials only following administration of massive amounts of metabolic precursors, e.g., retinol (18, 19), retinyl acetate (20, 21) or retinoic acid itself (22, 23). We present a highly sensitive quantitative determination for RA in human serum. Reference values range from 2.7 to 4.2 ng/ml and fit a normal-Gaussian distribution curve. EXPERIMENTAL
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